diff --git a/libs/ardour/test/testrunner.cc b/libs/ardour/test/testrunner.cc
index 41bc52393c..f30129332b 100644
--- a/libs/ardour/test/testrunner.cc
+++ b/libs/ardour/test/testrunner.cc
@@ -19,10 +19,6 @@ static const char* localedir = LOCALEDIR;
 int
 main(int argc, char* argv[])
 {
-	if (!Glib::thread_supported()) {
-		Glib::thread_init();
-	}
-
 	const struct option longopts[] = {
 		{ "debug", 1, 0, 'D' },
 		{ 0, 0, 0, 0 }
diff --git a/libs/audiographer/tests/test_runner.cc b/libs/audiographer/tests/test_runner.cc
index d378b5e4cb..594794d192 100644
--- a/libs/audiographer/tests/test_runner.cc
+++ b/libs/audiographer/tests/test_runner.cc
@@ -4,7 +4,6 @@
 
 int main()
 {
-	Glib::thread_init();
 	CppUnit::TextUi::TestRunner runner;
 	CppUnit::TestFactoryRegistry &registry = CppUnit::TestFactoryRegistry::getRegistry();
 	runner.addTest (registry.makeTest());
diff --git a/libs/pbd/pbd.cc b/libs/pbd/pbd.cc
index c19af3e462..7248ec7705 100644
--- a/libs/pbd/pbd.cc
+++ b/libs/pbd/pbd.cc
@@ -87,10 +87,6 @@ PBD::init ()
 	}
 #endif
 
-	if (!Glib::thread_supported()) {
-		Glib::thread_init();
-	}
-
 	Gio::init ();
 
 	PBD::ID::init ();
diff --git a/libs/pbd/test/rcu_test.cc b/libs/pbd/test/rcu_test.cc
index 36221281fe..7fb736cfac 100644
--- a/libs/pbd/test/rcu_test.cc
+++ b/libs/pbd/test/rcu_test.cc
@@ -15,9 +15,6 @@ CPPUNIT_TEST_SUITE_REGISTRATION (RCUTest);
 void
 RCUTest::setUp ()
 {
-	if (!Glib::thread_supported ()) {
-		Glib::thread_init ();
-	}
 }
 
 static void*
diff --git a/libs/pbd/test/signals_test.cc b/libs/pbd/test/signals_test.cc
index 27a2aa8359..61a0503b66 100644
--- a/libs/pbd/test/signals_test.cc
+++ b/libs/pbd/test/signals_test.cc
@@ -10,9 +10,6 @@ CPPUNIT_TEST_SUITE_REGISTRATION (SignalsTest);
 void
 SignalsTest::setUp ()
 {
-	if (!Glib::thread_supported ()) {
-		Glib::thread_init ();
-	}
 }
 
 class Emitter {
diff --git a/libs/temporal/test/testrunner.cc b/libs/temporal/test/testrunner.cc
index 9222e3caf0..cbecd687da 100644
--- a/libs/temporal/test/testrunner.cc
+++ b/libs/temporal/test/testrunner.cc
@@ -17,10 +17,6 @@
 int
 main(int argc, char* argv[])
 {
-	if (!Glib::thread_supported()) {
-		Glib::thread_init();
-	}
-
 	const struct option longopts[] = {
 		{ "debug", 1, 0, 'D' },
 		{ 0, 0, 0, 0 }